Sephadex - Wikipedia
Sephadex is used to separate molecules by molecular weight. Sephadex is a faster alternative to dialysis (de-salting), requiring a low dilution factor (as little as 1.4:1), with high activity recoveries.
Performing a Separation with Sephadex - MilliporeSigma
This page shows how to perform a separation with a Sephadex column from Cytiva which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.
Size Exclusion Chromatography | Cytiva
Size exclusion chromatography (SEC), also known as gel filtration, is the mildest of all the chromatography techniques. SEC separates molecules by differences in size as they pass through a resin packed in a column.
SephadexTM chromatography resin in 1959. SEC may be performed mainly in two principally different modes, depending on the size differences of the solutes to be sep. ting, buffer exchange, and sample cleanup. Sephadex resins are members of the BioProcessTM resin family and carry comprehensive techn.
Sephadex® is a beaded gel filtration medium prepared by cross-linking dextran with epichlorohydrin under alkaline conditions.1 General information and procedures for using gel filtration to separate proteins or to desalt protein solutions have been described.2,3. of double-stranded DNA fragments. The exclusion limits for d. G-25: 10 base pairs.
Sephadex G-100 Medium 9050-94-6 - MilliporeSigma
Sephadex ® is composed of repeating glucose units attached by α-1,6 glucosidic bonds. This structure ensures more aptamer binding sites and greater binding capacity. It is suitable for large-scale purification and is considered stable and can be utilized more than once.
Sephadex is a dry, bead-formed resin prepared by cross-linking dextran with epichlorohydrin. The resin swells in aqueous solutions. Sephadex ion exchangers are produced by introducing functional groups onto Sephadex. These groups are attached to glucose units in the matrix by stable ether linkages.