Size Exclusion Chromatography | Cytiva
Size exclusion chromatography (SEC), also known as gel filtration, is the mildest of all the chromatography techniques. SEC separates molecules by differences in size as they pass through a resin packed in a column.
Size-exclusion chromatography - Wikipedia
Size exclusion chromatography (SEC) is fundamentally different from all other chromatographic techniques in that separation is based on a simple procedure of classifying molecule sizes rather than any type of interaction.
separation mechanism is based on the size of the polymer molecules in solution. There are several names given to different types of SEC, but all are based on the same principle, that of size exclusion, hence size exclusion chromatography. Historically the porous medium was made of a gel and therefore gel permeation chromatography was coined, a term
Size Exclusion Chromatography (SEC) - SHIMADZU …
Size exclusion chromatography (SEC) allows compounds to be separated by differences in molecular size. Fig.1 shows the mechanisms of SEC. The packing material used in the column has many pores.
28.7: Size-Exclusion Chromatography - Chemistry LibreTexts
2023年1月24日 · Size-exclusion chromatography is carried out using conventional HPLC instrumentation, replacing the HPLC column with an appropriate size-exclusion column. A UV/Vis detector is the most common means for obtaining the chromatogram.
A brief practical review of size exclusion chromatography: Rules …
2018年10月1日 · Size exclusion chromatography (also frequently called gel filtration chromatography) is a fundamental tool in protein biochemistry. The first and most common application of SEC in protein biochemistry is as a fractionation step to separate macromolecules and other molecules on the basis of size.
SEC HPLC: Size Exclusion Chromatography - Phenomenex
Optimize your chromatographic separations with Phenomenex's SEC HPLC columns. Explore superior Size Exclusion Chromatography for precise molecular size analysis.
Size Exclusion-High-Performance Liquid Chromatography (SEC-HPLC…
2014年1月1日 · The size exclusion-high-performance liquid chromatography is a high-throughput analytical method, through isocratic condition, that allows to determine and quantify the level of aggregates and fragments of purified antibodies.
High-performance liquid chromatography - Wikipedia
High-performance liquid chromatography (HPLC), formerly referred to as high-pressure liquid chromatography, is a technique in analytical chemistry used to separate, identify, and quantify specific components in mixtures. ... Size-exclusion chromatography (SEC) [30] ...
Size-Exclusion Chromatography for the Analysis of Protein ...
While numerous techniques have been developed to monitor protein aggregation, size-exclusion chromatography (SEC) has been predominantly favored for routine and validated analyses because of both its speed and reproducibility. [3 – 6] SEC is also an accurate method if confirmed with an orthogonal method, such as sedimentation velocity analytical...