FabALACTICA - Genovis
FabALACTICA (IgdE) is a cysteine protease that digests human IgG1 at a specific site above the hinge, generating intact and homogenous Fab and Fc fragments. The enzyme facilitates the characterization of bi- and multispecific antibodies and analyses of …
Novel IgG-Degrading Enzymes of the IgdE Protease Family Link
2016年10月17日 · The novel IgdE family proteases of S. agalactiae, S. pseudoporcinus and S. equi showed IgG subtype specificity, i.e. IgdE from S. agalactiae and S. pseudoporcinus cleaved human IgG1, while IgdE from S. equi was subtype specific for equine IgG7.
Identification and Characterization of IgdE, a Novel IgG-degrading ...
2016年4月8日 · This enzyme, designated IgdE forimmunoglobulinG-degradingenzyme ofS. suis, is a cysteine protease distinct from previous characterized streptococcal immunoglobulin degrading proteases of the IdeS family and mediates efficient cleavage of the hinge region of porcine IgG with a high degree of specificity.
IgdE family proteases cleave IgG in the hinge region. (A) The …
IgdE, derived from Streptococcus agalactiae, is a protease optimised for the cleavage of the hIgG1. 10 Although hIgG1 represents about 60-66% of total hIgG, the amount of intact IgG detected...
Novel IgG-Degrading Enzymes of the IgdE Protease Family Link
2016年10月17日 · The novel IgdE family proteases of S. agalactiae, S. pseudoporcinus and S. equi showed IgG subtype specificity, i.e. IgdE from S. agalactiae and S. pseudoporcinus cleaved human IgG1, while IgdE from S. equi was subtype specific for equine IgG7.
Full article: Characterization and analysis of scFv-IgG bispecific ...
2018年4月19日 · IgdE is a cysteine protease that cleaves the heavy chains above the hinge disulfide bonds (…KSCDKT/HTCPPC…), yielding two identical Fabs and one Fc fragment. After IgdE digestion, samples were treated with PNGase F and CpB to reduce heterogeneity arising from N-glycosylation and C-terminal lysine.
Identification and Characterization of IgdE, a Novel IgG-degrading ...
The inhibition of enzymatic activity with iodoacetamide and Z-LVG-CHN 2 and most importantly the lack of enzymatic activity of the IgdE C302S and IgdE H333A mutant variants strongly suggest that IgdE is a novel and so far unique member of the cysteine protease family.
2023年12月18日 · Here, we explore an alternative IgG1 hinge-cleaving protease, BdpK (FabDELLO), and compare it directly to IgdE for use in IgG1 repertoire profiling. We used IgdE and BdpK in parallel to digest all IgG1s from the same set of plasma samples.
Middle-up analysis of monoclonal antibodies after combined IgdE …
2018年2月5日 · In this study, we introduced the use of a new enzyme (IgdE) that cleaves mAbs with high specificity at their upper hinge region in non-reducing conditions to investigate their disulfide bridge oxidation state.
Direct Comparison of the Hinge-Cleaving Proteases IgdE and …
2023年12月17日 · IgdE and BdpK have adjacent albeit distinct cleavage sites, whereby BdpK cleaves one Threonine amino acid closer to the N-terminus of IgG1 compared to IgdE. Here, we set out to evaluate whether also BdpK can be used for plasma IgG1 clonal profiling, benchmarking it directly versus IgdE.
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