High Pressure Size Exclusion Chromatography (HPSEC) …
We reassessed the molecular weight of dissolved organic matter (DOM) determined by high pressure size exclusion chromatography (HPSEC) using measurements made with different columns and various generations of polystyrenesulfonate (PSS) molecular weight standards.
Size-exclusion chromatography - Wikipedia
Size-exclusion chromatography, also known as molecular sieve chromatography, [1] is a chromatographic method in which molecules in solution are separated by their shape, and in some cases size. [2] . It is usually applied to large molecules or macromolecular complexes such as proteins and industrial polymers. [3] .
An overview of the uses of high performance size exclusion ...
2019年2月1日 — HPSEC is a useful separation technique that has found uses in NOM weight fractionation, and operates similar to other separation techniques such as vapor pressure osmometry (VPO) or asymmetrical flow field-flow fractionation (AF4) (Pifer and Fairey, 2012; Neubauer et al., 2013; Pelekani et al., 1999). HPSEC operates by allowing the operator to ...
Size-Exclusion Chromatography for the Analysis of Protein ...
For these largely protein-based therapies, the quantitation of aggregates is of particular concern given their potential effect on efficacy and immunogenicity. This need has renewed interest in size-exclusion chromatography (SEC).
High-performance size-exclusion chromatography of peptides
2003年6月30日 — High-performance size-exclusion chromatography (HPSEC) employs pre-packed columns (internal diameter: 0.45–1 cm, length: 25–60 cm) containing small particles (diameter of 15 μm or less), often silica-based, resulting in tens of …
Thorough Validation of Optimized Size Exclusion Chromatography …
2024年4月30日 — Size exclusion chromatography with total organic carbon detection (HPSEC-TOC) is a widely employed technique for characterizing aquatic natural organic matter (NOM) into high, medium, and low molecular weight fractions.
Calibration of high performance size exclusion chromatography (HPSEC …
2022年12月1日 — Three high performance size exclusion chromatography (HPSEC) columns were compared, along with mobile phase chemistry and calibration standards, for the determination of apparent molecular weight (AMW) of aquatic humic substances using a …
An overview of the uses of high performance size exclusion ...
This review mainly focuses on the use of HPSEC in the characterization of NOM in a potable water setting, with an additional focus on strong-base ion-exchangers specifically targeted for NOM constituents.
Size Exclusion-High-Performance Liquid Chromatography (SEC …
2014年1月1日 — The size exclusion-high-performance liquid chromatography is a high-throughput analytical method, through isocratic condition, that allows to determine and quantify the level of aggregates and fragments of purified antibodies. Here, we describe the preparation of the...
Development and validation of high-performance size exclusion ...
2014年5月15日 — As demonstrated, these HPSEC methods are rapid, accurate, and reproducible for the polysaccharides and their glycoconjugates and provide a relevant and more informative alternative to the current CGPC methods.