We investigated whether T cells can promote IFN-α production by pDCs. Methods Human pDCs were stimulated with immune complexes (ICs) containing U1 small nuclear ribonucleic proteins particles and ...

Herpesviruses are common co-pathogens in individuals infected with human immunodeficiency virus (HIV). Herpes simplex virus ...

This valuable study reveals extensive binding of eukaryotic translation initiation factor 3 (eIF3) to the 3' untranslated regions (UTRs) of efficiently translated mRNAs in human pluripotent stem ...

Lactate dehydrogenase (LDH) release was used to identify non-toxic concentrations of quercetin. IL-6, IL-8, IL-1α, IL-1β and tumour necrosis factor alpha (TNFα) proteins were measured in tissue ...

This important manuscript provides insights into the competition between Splicing Factor 1 (SF1) and Quaking (QKI) for binding at the ACUAA branch point sequence in a model intron, regulating exon ...

Methods—The secretion of TNF-α, TNF-β, and soluble TNF receptors was compared in the culture supernatants of colonic biopsy specimens and isolated lamina propria mononuclear cells from patients with ...

IFN-α inhibition assays were conducted using PBMCs cocultured with the TLR9 agonist and drugs. The IFN-α in the supernatants was detected using homogeneous time-resolved fluorescence method.

Culture supernatants and whole sera can also be analysed. Important: To label proteins, please avoid using fluorophores which have emissions similar to the wavelength of Cy3 which we incorporate into ...

1 Department of Cell Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, USA 2 Department of Biological Sciences, Cleveland State University, Cleveland, Ohio, USA 3 Department of ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. During PINK1- and Parkin-mediated mitophagy, ...