Set aside 1–2 μl from each sample to compare the extracted chromatin with matched sonicated samples by gel electrophoresis. Sonicate the resuspended chromatin solution five times for 15 s each ...
This important manuscript provides insights into the competition between Splicing Factor 1 (SF1) and Quaking (QKI) for binding at the ACUAA branch point sequence in a model intron, regulating exon ...
Before use, PFFs should be sonicated to a length of 50 nm or shorter to ensure their recruiting activity and facilitate endocytosis. The morphology and activity of PFFs can be verified using an ...