Heterokaryon - Wikipedia
网页A heterokaryon is a multinucleate cell that contains genetically different nuclei. Heterokaryotic and heterokaryosis are derived terms. This is a special type of syncytium.
Heterokaryon - an overview | ScienceDirect Topics
网页Heterokaryons. When two compatible vegetative cultures of Neurospora are allowed to grow together, they may fuse to form a single individual (mycelium) containing nuclei from both contributing strains. Such “heterokaryons” use gene products from both components and are useful to test for genetic complementation.
Heterokaryon - an overview | ScienceDirect Topics
网页An approach conceptually related to the reprogramming of somatic nuclei following their introduction into the cytoplasm of a Xenopus egg or oocyte, is the fusion of two distinct somatic cells to form a single cell with two different nuclei bathed in a …
Heterokaryon - an overview | ScienceDirect Topics
网页Genetically distinct strains can fuse to produce hyphae containing variable mixtures of nuclei with different genotypes called heterokaryons. Because there is no mechanism for maintaining consistent ratios of the two types of nuclei, heterokaryons are …
A Quantitative Heterokaryon Assay to Measure the …
网页2018年9月9日 · Here, we present a quantitative assay where stable donor cell lines expressing near-physiological levels of eGFP-tagged RBPs are fused to recipient cells expressing the membrane marker CAAX-mCherry, allowing to readily identify and image a large number of high-confidence heterokaryons.
Using heterokaryons to understand pluripotency and …
网页2011年8月8日 · In our studies, mouse ES cells and human B cells were pre-labelled with different cell-surface dyes, mixed together and fused with polyethylene glycol. Heterokaryons containing a human and a mouse nucleus were isolated by fluorescence-activated cell sorting of double-labelled cells.
Nuclear interactions in a heterokaryon: insight from the model ...
网页2014年7月7日 · A heterokaryon is a tissue type composed of cells containing genetically different nuclei. Although heterokaryosis is commonly found in nature, an understanding of the evolutionary implications of this phenomenon is largely lacking.
A Quantitative Heterokaryon Assay to Measure the ... - PubMed
网页2018年9月5日 · Here, we present a quantitative assay where stable donor cell lines expressing near-physiological levels of eGFP-tagged RBPs are fused to recipient cells expressing the membrane marker CAAX-mCherry, allowing to readily identify and image a large number of high-confidence heterokaryons.
Nuclear-specific gene expression in heterokaryons of the …
网页2022年8月10日 · Heterokaryon expression is predominantly defined by additive effects of its two nuclear components. Furthermore, allele-specific expression analysis of heterokaryons with varying nuclear ratios show patterns of expression ratios strongly dependent on nuclear ratios in the vegetative stage.
Heterokaryons: An Assay for Nucleocytoplasmic Shuttling
网页Interspecies heterokaryons, which constitute the most widely used shuttling assay, involve the formation of hybrid cells containing at least two nuclei from different species.
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